Table 1.
B. subtilis inherent terpenoid biosynthesis enzymes
| Inherent pathways | EC number | Strains |
|---|---|---|
| Mevalonate pathway | 2.3.1.9 |
Bacillus subtilis subsp. Subtilis 168 Bacillus subtilis subsp. Subtilis RO-NN-1 Bacillus subtilis subsp. Subtilis BSP1 Bacillus subtilis subsp. Subtilis 6051-HGW Bacillus subtilis subsp. Subtilis BAB-1 Bacillus subtilis subsp. Subtilis AG1839 Bacillus subtilis subsp. Subtilis JH642 Bacillus subtilis subsp. Subtilis OH 131.1 Bacillus subtilis subsp. spizizenii W23 Bacillus subtilis subsp. spizizenii TU-B-10 Bacillus subtilis subsp. Natto BEST195 Bacillus subtilis BSn5 Bacillus subtilis QB928 Bacillus subtilis XF-1 Bacillus subtilis PY79 |
| MEP/DOXP pathway | 2.2.1.7, 1.1.1.267, 2.7.7.60, 2.7.1.148, 4.6.1.12, 1.17.7.1, 1.17.1.2, 5.3.3.2 | |
| Terpenoid backbone biosynthesis (downstream) | 2.5.1.1, 2.5.1.10, 2.5.1.29, 2.5.1.30, 2.5.1.31 | |
| Ubiquinone and other terpenoid-quinone biosynthesis | 2.5.1.74, 2.1.1.163, 2.5.1.- | |
| Carotenoid biosynthesis | 2.5.1.32 |
• Detailed information can be found at KEGG website, http://www.kegg.jp/
• Underlined enzymes (B. subtilis): functional parameters can be found at the BRENDA website, http://brenda-enzymes.info/index.php
• 2.3.1.9, acetyl-CoA acetyltransferase, yhfS
• 2.2.1.7, 1-deoxy-D-xylulose-5-phosphate synthase, dxs
• 1.1.1.267, 1-deoxy-D-xylulose 5-phosphate reductoisomerase, dxr
• 2.7.7.60, 2-D-methyl-D-erythritol 4-phosphate cytidylyltransferase, ispD
• 2.7.1.148, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, ispE
• 4.6.1.12, 2-D-methyl-D-erythritol 2,4-cyclodiphosphate synthase, ispF
• 1.17.7.1, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase, ispG
• 1.17.1.2, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, ispH
• 5.3.3.2, isopentenyl-diphosphate delta-isomerase, idi
• 2.5.1.1, 2.5.1.10, 2.5.1.29, geranylgeranyl diphosphate synthase, type II, ispA
• 2.5.1.30, heptaprenyl diphosphate synthase component 2, hepT
• 2.5.1.31, undecaprenyl diphosphate synthase, uppS
• 2.5.1.74, 2.5.1.-, 1,4-dihydroxy-2-naphthoate octaprenyltransferase, menA
• 2.1.1.163, demethylmenaquinone methyltransferase, ubiE
• 2.5.1.32, phytoene synthase, crtB