Table 2.
Detection and analysis reports of B. subtilis terpenoid pathway metabolites
| Method | Compound | Characteristic | Reference |
|---|---|---|---|
| GC-MS | Isoprene | Rt = 16.5 min, m/z 39, 53, 67 | Kuzma et al. 1995 |
| GC | Isoprene | Wagner et al. 1999 | |
| GC-MS (13C, 2H labeling) | Isoprene | Common substrate m/z 39, 53, 67; Substrate: U-[13C6] glucose m/z 42, 57, 72; 1-[13C] pyruvate m/z 40, 54, 68; 2-[13C] pyruvate m/z 40, 55, 69; 3-[13C] pyruvate m/z 40, 54, 69. |
Wagner et al. 2000 |
| GC | DMAPP | Fisher et al. 2001 | |
| GC | Isoprene | Rt = 2.6 min | Shirk et al. 2002 |
| HPLC | Acetoin | Rt = 5.5 min, 354 nm | |
| Kits | Glucose | ||
| Kits | Lactic, pyruvic acids | ||
| CIMS | (M + H)+(H2O)n: | Custer et al. 2003 | |
| Acetaldehyde | m/z 63 (n = 1) | ||
| Acetoin | m/z 89 (n = 0) | ||
| Acetone | m/z 77 (n = 1) | ||
| 2,3-Butanediol | m/z 91 (n = 0) | ||
| Butanol | m/z 111 (n = 2) | ||
| 2-Butanone | m/z 91 (n = 1) | ||
| Butyraldehyde | m/z 91 (n = 1) | ||
| Butyl acetate | m/z 135 (n = 1) | ||
| Diacetyl | m/z 123 (n = 2) | ||
| Dimethyl sulfide | m/z 63 (n = 0) | ||
| Ethanol | m/z 83 (n = 2) | ||
| Ethyl acetate | m/z 107 (n = 1) | ||
| Isoamyl alcohol | m/z 107 (n = 1) | ||
| Isoprene | m/z 69 (n = 0) | ||
| GC | Isoprene | Julsing et al. 2007 | |
| HPLC | 4,4′-Diapolycopene | Rt = 26.8 min, Absorption: 293, 443, 472, 501 nm | Yoshida et al. 2009 |
| 4,4′-Diaponeurosporene | Rt = 28.9 min, Absorption: 266, 415, 439, 469 nm | ||
| MALDI-TOF MS | 4,4′-Diapolycopene | m/z 399.9 | |
| 4,4′-Diaponeurosporene | m/z 401.9 | ||
| GC-MS | Isoprene | Rt = 1.9 min | Xue and Ahring 2011 |
| HPLC | Glycosyl 4,4′-diaponeurosporenoate | Rt = 10.0 min, Absorption: 282, 469 nm | Barredo 2012 |
| 4,4′-Diapolycopene | Absorption: 293, 443, 472, 501 nm | ||
| 4,4′-Diaponeurosporene | Rt = 14.4 min, Absorption: 265, 414, 441, 469 nm | ||
| UPLC-MS | DXP | Rt = 5.6 min, m/z 213.0170 | Tsuruta et al. 2009; Zhou et al. 2012; Zhou et al. 2013a |
| MEP | Rt = 5.2 min, m/z 215.0330 | ||
| CDP-ME | Rt = 6.2 min, m/z 520.0730 | ||
| CDP-MEP | Rt = 7.3 min, m/z 600.0390 | ||
| MEC | Rt = 6.6 min, m/z 276.9884 | ||
| HMBPP | Rt = 7.0 min, m/z 260.9920 | ||
| GC-MS | Trans-Caryophyllene | Rt = 3.4 min, m/z 189, 204 | |
| Amorpha-4,11-diene | Rt = 3.5 min, m/z 189, 204 | ||
| LC-Fourier transform MS | (Untargeted metabolomics study) | Cho et al. 2014 | |