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Polymerase chain reaction Detection of Clostridia in shoots of inoculated plants. (A,B) PCR with 10 ng of DNA isolated from shoot after growth in soil. (A) PCR with P930/P932 (665 bp, 16S-rDNA). (B) PCR with F2/R2 (462 bp, BoNT D) and F4/R4 (128 bp, BoNT D).
