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. 2015 Oct 31;6:1217. doi: 10.3389/fmicb.2015.01217

FIGURE 3.

FIGURE 3

Accumulation of ROS/RNS as detected by CM-H2DCFDA in cultures of Synechococcus 7002 wild type and SYNPCC7002_A1649 (fur), SYNPCC7002_A1836 and SYNPCC7002_A2498 mutant strains exposed to high light. Cultures of wild type Synechococcus 7002 and SYNPCC7002_A1649 (fur), SYNPCC7002_A1836, and SYNPCC7002_A2498 mutant strains were grown to OD730 nm = 0.7 under standard conditions, and then CM-H2DCFDA was added and the cultures were exposed to high light (830 μmol photons m-2 s-1). Samples were taken from two independent cultures and measured in duplicate after 0 h, 1 h, 2 h, and 3 h of high light incubation. The cultures were adjusted to OD730 nm = 0.5 for subsequent fluorescence emission measurements.