Table 1.
Oligonucleotide primers used in this study.
| Number | Sequence (relevant restriction sites underlined, 5′ to 3′) |
|---|---|
| #1 | AAATCTAGAGCAATGGTGCTAATCTCCTC (XbaI site underlined) |
| #2 | TTTGATATCATATTTTTCAACAAGTTCAG (EcoRV site underlined) |
| #3 | AAAAAGCTTAATCGAGGCGATCGCATTTGACGGTAAC (HindIII site underlined) |
| #4 | TTTCTCGAGGCACTCCGCAGGGCTAAATC (XhoI site underlined) |
| #5 | AAATCTAGACCGCAATCTGGGTGCCGAGG (XbaI site underlined) |
| #6 | TTTGATATCAAGGCTTGTTCGTTGCAAAATC (EcoRV site underlined) |
| #7 | AAAGATATCTAACTTGTTTAAATAATCTG (EcoRV site underlined) |
| #8 | TTTCTCGAGGGGTATCGCTTTGGGCACAG (XhoI site underlined) |
| #9 | AAATCTAGATGATGGCGGGAATCCTCGTC (XbaI site underlined) |
| #10 | TTTGATATCATTAGGCTTCTGGTTGGGTG (EcoRV site underlined) |
| #11 | ACCCAAGCTTCAGGCCGATAATGCTGAATG (HindIII site underlined) |
| #12 | TTTCTCGAGATATAGGAGTGGCCATCCTC (XhoI site underlined) |
| #13 | AAATCTAGAACTGCTCGCCGCCGTGGAACAG (XbaI site underlined) |
| #14 | CCCCCCGGGCATTAAATCAAAAATAATTGATGC (SmaI site underlined) |
| #15 | AAAAAGCTTAAAACCCCAACCCAGAATATAAATC (HindIII site underlined) |
| #16 | CCCCTCGAGAGCTGCATCTTCATAGGTAAATCCC (XhoI site underlined) |
| #17 | TGCTCTAGACAAGGGCAATATGAACTGGC (XbaI site underlined) |
| #18 | TCCGATATCTTGGCATCCCCCAAGACACC (EcoRV site underlined) |
| #19 | CCCAAGCTTACACACCCACTAATCAACGC (HindIII site underlined) |
| #20 | CCGCTCGAGAAGGTTACGAAGTTCATGGC (XhoI site underlined) |