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. 2015 Oct 31;6:838. doi: 10.3389/fpls.2015.00838

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Copyright © 2015 Bajwa, Shahid, Rao, Bashir, Aftab and Husnain.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Complete protocol of agrobacterium mediated transformation in cotton plant embryos. (A) germinated cotton seeds, (B) isolation and injuring of cotton embryos, (C) Agrobacterium treated embryos on MS medium, (D) Agrobacterium treated embryos on MS medium after 3 days, (E) implantation of cotton embryos in test tube containing MS medium with kanamycin, (F) shoot growing on MS medium containing kanamycin selection, (G) root development on MS rooting medium, (H) transgenic cotton plants shifted to soil for acclimatization.