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. 2015 Oct 31;6:838. doi: 10.3389/fpls.2015.00838

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Copyright © 2015 Bajwa, Shahid, Rao, Bashir, Aftab and Husnain.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PCR amplification of transgenic cotton plants during three generations (Fiber Gene GhEXPA8). (A) represented the PCR amplification of transgenic cotton plants at T₀ generation, lane 1: 1 kb DNA marker, lane 2–7: transgenic cotton plants, Lane 8: Negative Control, Lane 9: Positive Control; (B) PCR amplification of transgenic cotton plants during T₁ generation, lane 1: 1 kb DNA marker, lane 2: negative control, lane 3–8: transgenic cotton plants; (C) During T₂ generation Lane 1–8 show putative transgenic plants in which lane 1, 2, 3, 4, 6, and 8 show positive transgenic plants having gene amplification from orientation primers resulting in band of 960 bp whereas, Lane 5 and 7: untransformed cotton (negative amplification), Lane 9: DNA from untransformed cotton (Negative Control), Lane 10: DNA from pGA482-GhEXPA8 (Positive Control), lane 11:1 kb DNA ladder Whereas, the arrow indicate the detection of gene in transgenic cotton plants.