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. 2015 Oct 31;6:838. doi: 10.3389/fpls.2015.00838

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Copyright © 2015 Bajwa, Shahid, Rao, Bashir, Aftab and Husnain.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PCR analysis of transformed cotton for NPTII gene, 35S CaMV promoter region, and VirG gene. (A) (NPTII gene): Lanes 2–7: putative transgenic cotton lines; lane 8: untransformed cotton, lane 9: pGA482-GhEXPA8; lane 1: 50 bp DNA ladder (Fermentas, USA). (B) (35S CaMV promoter region): lane 1: 100 bp DNA ladder (Fermentas, USA) Lanes 2–7: putative transgenic cotton lines; lane 8: untransformed cotton, lane 9: pGA482-GhEXPA8. (C) (VirG gene of Agrobacterium): lane 1: 1 kb DNA ladder (Fermentas, USA) Lanes 2–7: putative transgenic cotton lines; lane 8: untransformed cotton, lane 9: pGA482-GhEXPA8.