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. 2015 Oct 31;6:838. doi: 10.3389/fpls.2015.00838

Figure 4.

Figure 4

Determination of copy number of transgene (Fiber Gene GhEXPA8) through southern blot analysis. Lane M: 1 kb DNA Ladder, Lane 1: Negative Control (DNA of untransformed plant), Lane 2–7 (FG-8-1, FG-8-4, FG-8-5, FG-8-11, FG-8-13, and FG-8-15 respectively): Selected Transgenic Cotton Plant Samples (pGA482-GhEXPA8) were digested with EcoR1, probed with biotin-labeled GhEXPA8 DNA to assess copy number of the transgene in the transformed plants. The number of bands revealed that the plant FG-8-15 had one copy of the transgene in its genome, FG-8-1, FG-8-4, and FG-8-13 had two copies of the transgene in its genome, whereas the plants FG-8-5 and FG-8-11 had three copies of the transgene. Figure determined 1.56 kb highlighted region along with other highlighted region which gives information about the copy number. Lane 1 contains 1 Kb ladder, lane 2 represented negative controls, in lane 3–8 indicate the integration of fiber gene GhEXPA8 in selected transgenic cotton plant samples.