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. 2015 Oct 30;15:248. doi: 10.1186/s12866-015-0588-0

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© Perdoni et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Ultrastructural imaging of A. fumigatus Af293 biofilm morphology treated or not with Myriocin (4 mg/L). a 48h untreated biofilm: normal fungal cells morphology (12000X original magnification -o.m.; Bar = 600 nm); b 4h post-Myriocin: the fungal cell showed some alterations of the inner membrane that became non homogeneous (12000X o.m.; Bar = 600 nm); c 24h post-Myriocin: the plasma membrane showed several disorganized invaginations associated with cell wall detachment (20000X o.m.; Bar = 400 nm); d 24h post-Myriocin: the hyphae showed a cell wall collapsed, and a degenerated cytoplasm highly vacuolarized; none of the cell organelles was recognizable. (12000X o.m.; Bar = 600 nm); e 48h post-Myriocin: a large part of the fungal cells is damaged and disrupted (12000X o.m.; Bar = 2.5 μm)