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Expression of nrf2 protein in chicken hepatocytes. The cells were pre-treated for 24 h with different concentrations of PEF ranging from 0 to 40 μg/ml and positive control (gallic acid, 10 μM or 1.7 μg/ml). The media were replaced with a medium containing 5 μM of AFB1 and the cells were incubated for another 48 h. All values represent means ± SEM from three independent experiments. *** p < 0.001 and ** p < 0.01 indicate significant difference compared to the untreated control (0)
