Table 4.
Zebrafish and mouse predicted enhancers subjected to experimental validation
| ECR | Zebrafish (Zv9 July 2010) | Mouse (mm9) July 2007) | ||
|---|---|---|---|---|
| Expression | Coordinates | Expression | Coordinates | |
| 1 | Spinal cord (48) | chr6:20,797,116-20,798,106 | negative | chr5:107,545,775–107,546,750 |
| 2 | Epidermis (24,48) | chr6:43,538,446–43,539,094 | negative | chr6:98,881,656–98,882,625 |
| negative | chr6:98,883,956–98,884,889 | |||
| 3 | Tail bud (24), eye (48), heart (48) | chr9:11,657,708–11,658,704 | not tested | |
| 4 | Notochord (24,48) | chr19:22,713,465–22,714,026 | negative | chr18:80,876,694–80,877,675 |
| negative | chr18:80,876,778–80,877,739 | |||
| 5 | Epidermis (24,48) | chr19:41,272,614–41,273,241 | not tested | |
| 6 | Epidermis (24,48), Otic Vesicle (24,48), Somitic muscle (24,48) | chr2:39,790,777–39,791,782 | Epidermis (24,48), Notochord (24), Olfactory epithelium (24,48), Spinal cord (24), Otic Vesicle (48), Pectoral fin (48) | chr1:77,387,974–77,389,004 |
| 7 | Hindbrain (24,48), Olfactory epithelium (24,48) | chr2:39,792,312–39,793,241 | Eye (24,48), Olfactory bulb (24,48), Hindbrain (24,48) | chr1:77,390,034–77,391,031 |
| 8 | Somitic muscle (24,48), Olfactory epithelium (48), Hindbrain (48) | chr2:39,837,096–39,838,009 | negative | chr1:77,503,689–77,504,676 |
| negative | chr1:77,506,344–77,507,445 | |||
| 9 | Spinal cord (24,48) | chr4:16,470,975–16,471,819 | Midbrain (24), Yolk (24) | chr10:87,004,979–87,005,964 |
| Somitic Muscle (24,48), Skin under yolk (24,48), Phar. Arches (48), Pectoral fin (48) | chr10:87,008,830–87,009,792 | |||
| 10 | Somitic muscle (24,48), Olfactory epithelium (48) | chr9:22,365,926–22,366,799 | Somitic Muscle (24,48), Pericardium (24,48) | chr14:58,234,894–58,235,874 |
| 11 | Hindbrain (24), Somitic muscle (24,48), Heart (48) | chr9:22,368,460–22,369,405 | ||
| 12 | Epidermis (24,48), Otic vesicle (24,48), Yolk (24,48) | chr14:41,645,428–41,646,386 | Epidermis (24), Olfactory epithelium (24) | chrX:130,162,955–130,163,923 |
| 13 | Hindbrain (24), Olfactory epithelium (24,48), Line under eye (24,48), Spinal cord (48) | chr20:21,970,078–21,970,911 | Spinal cord (24) | chr12:72,999,373–73,000,279 |
Tissues in bold (in ECR 6, 7, 10, 11, 12) show consistent enhancer activity between the mouse and zebrafish syntenic enhancers at the indicated time point (24 or 48 hpf). Each construct was injected in at least 100 embryos; the proportion of fish alive and expressing in a given tissue is shown in Additional file 2: Table S1