Fig. 6.

Cross-sections of ‘Braeburn’ microstructure illustrating consecutive steps of the cell isolation and void characterization protocol. The images obtained by means of X-ray CT are cropped and filtered before segmentation into the cellular matrix and void network. These complementary binary images are processed in a separate workflow. Voids intersecting the border of the image are removed and resulting voids are characterized in 3D. The cellular matrix is separated in individual objects by the watershed separation algorithm. Objects touching the image border are removed and remaining objects are measured. Objects of which the length and sphericity do not fall within the user-defined range of cellular characteristic are removed. The analyzed void network and separated cells are overlayed with the original image. Scale bars in images indicate 250 μm