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. 2015 Oct 31;12:199. doi: 10.1186/s12974-015-0419-0

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© Chuang et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Determination of primary culture purity after isolation from brains of postnatal day 7–10 c57bl/6 mice. a Flow cytometry of cells from pre-sorting showed 14.43 % of cells in cell suspension positive for cd11b-FITC. b Flow cytometry of cells from post-sorting showed 90.61 % of cells in cell suspension positive for cd11b-FITC. c Immunocytochemical staining of cells in DIV5 primary culture showed expression of cd11b (red) in almost all cells