Table 2.

Primers used in this study

Virus	Forward primer sequence (5′−3′)	Reverse primer sequence (5′−3′)	Amplicon size (bp)	Target region
AIV-M	AGGTGACACTATAGAATA CAGAAACGGATGGGAGTGC	GTACGACTCACTATAGGGA TATCAAGTGCAAGATCCCAATGAT	122	M
AIV-H5	AGGTGACACTATAGAATA CTTCAGGCATCAAAATGCACA	GTACGACTCACTATAGGGA TAGTTTGTTCATTTCTGAGTCGGTC	285	HA
AIV-H7	AGGTGACACTATAGAATA AATGGGGCHTTCATAGCTCC	GTACGACTCACTATAGGGA TGATAGCARTCRCCTTCACAA	144	HA
AIV-H9	AGGTGACACTATAGAATA ACAACAAGTGTGACAACAGAAGA	GTACGACTCACTATAGGGA TCTTCCGTGGCTCTCTCC	237	HA
DHV	AGGTGACACTATAGAATA TCTTCGTTGTGAAACGGATTACC	GTACGACTCACTATAGGGA TGCCTGGACAGATDTGTGCCTACT	133	5′ UTR
DTMUV	AGGTGACACTATAGAATA ATGGACAGGGTCATCAGCGG	GTACGACTCACTATAGGGA GAATRGCTCCYGCCAATGCT	176	E
EDSV	AGGTGACACTATAGAATA AATCGGCAACTCAAGACATC	GTACGACTCACTATAGGGA CCCATTCATAAACAGGATTC	208	Penton
DEV	AGGTGACACTATAGAATA GGGAGGAGCAAACAAAGA	GTACGACTCACTATAGGGA ATCGCAAATTCCATCACATA	150	UL6
NDV	AGGTGACACTATAGAATA GTRGCAGCAAGRACAAGG	GTACGACTCACTATAGGGA CATATCYGCATACATCAA	196	L
DuCV	AGGTGACACTATAGAATA TGCKCCAAAGAGTCGACATA	GTACGACTCACTATAGGGA CAAAYGCATAACGGCTCTTTCC	300	Red
MDRV	AGGTGACACTATAGAATA CAGTTGAGCCGGAYGGTAATT	GTACGACTCACTATAGGGA ACTCGGTTGGTGTTAGTVGCVTAGAA	219	S1
MDPV	AGGTGACACTATAGAATA CTTTCAGGCTACATCTTCAA	GTACGACTCACTATAGGGA AATTCTCTTTTCACCCATCC	253	VP1
Universal tag sequences Tag-F: AGGTGACACTATAGAATA Tag-R: GTACGACTCACTATAGGGA

Universal tag sequences are underlined. Bold type indicates degenerate sites

Abbreviations: M A or C, R A or G, W A or T, S G or C, Y C or T, K G or T, V A, G, or C, H A, C, or T, D A, G, or T; B G, C, or T