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. 2015 Oct 31;6:209. doi: 10.1186/s13287-015-0204-0

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© Schiavo et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Cell isolation from collected amniotic fluid (from the second and third trimesters) and characterization by flow cytometry analysis. a Representative scheme of amniotic fluid retrieval for cell extraction from second-trimester amniocentesis (upper part) and cesarean section at term of pregnancy (below). Flow cytometry phenotyping revealed the variable presence of CD117 marker on freshly isolated cells (three-dimensional histograms, n = 10, isotype control in gray). b Representative dot-plot cytograms showing cells from both trimesters (second trimester in orange and third trimester in green) comparing various antigens (vertical axes) versus CD117 expression (horizontal axes)