Fig. 7.

ATZ affects the number of H3K4me3 marks and mRNA levels of genes involved in androgen signaling and testosterone metabolism. a The differential H3K4me3 peaks in the Ncoa2 gene (there are two regions with changes in H3K4me3 marks outlined in boxes), b Cyp19a1 gene and c Star gene. The Y-axis presents the plotted values of tags at each position after normalizing to all reads in the same dataset (intensity range shown on the left side of the plot); the X-axis shows the schema of the gene; rectangles are exons, and lines are introns. Note that the differential regions are located within genes. The H3K4me3 profiles from control biological replicates are shown in red, and those from ATZ-treated mice are in blue. For illustration, the “Integrative Genomic Viewer” (IGV) software version 2.3.36 was used. d qPCR analysis of the Ncoa2, Cyp19a1, and Star genes. qPCR analysis of RNA samples was performed in three independent experiments. Primer sequences for each gene are shown in the Materials and Methods section. The data are presented as fold change in treated samples compared to controls, *p < 0.05, **p < 0.01