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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Arch Pathol Lab Med. 2015 Jul 14;139(11):1349–1361. doi: 10.5858/arpa.2014-0471-OA

Figure 4.

Figure 4

Higher microRNA-375 expression does not reduce tyrosine phosphorylation of cortactin in UMSCC1 cells. A, Representative Western blots (WBs) for cortactin in total phosphotyrosine immunoprecipitates (IP pTYR) compared to the total cell lysate (5% input) and nonspecific rabbit immunoglobin G (IP Rabbit IgG) and beads (Beads only) control. B, Quantitation of cortactin levels in immunoprecipated phosphotyrosine proteins, shown as mean and standard error of the mean (SEM) of 4 independent experiments. C, Representative WBs for cortactin and phosphocortactin (pY421) in cortactin immunoprecipitates (IP Cortactin) compared to the total cell lysate (5% input) and nonspecific mouse IgG (IP Mouse IgG) and beads (Beads only) controls. D, Quantitation of phosphocortactin levels compared to total cortactin in cortactin immunoprecipitates, shown as mean and SEM of 4 independent experiments. Abbreviation: n.s., not significant.