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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Insect Biochem Mol Biol. 2015 Jul 8;65:20–27. doi: 10.1016/j.ibmb.2015.07.004

Fig. 2.

Fig. 2

Transgene expression. Total RNA was isolated from the PSGs dissected from 5th instar w1-pnd or transgenic (Flc-GG#1 and Flc-GG#2) silkworm larvae and used for RT-PCRs, as described in Materials and methods. Each reaction was performed with (RT+) and without (RT−) reverse transcriptase to assess DNA contamination of the RNA preparations. One reaction was performed with no template to assess DNA contamination of our other reagents. The primer sets used for these RT-PCRs were specific for the endogenous BmRPL3 or human MGAT2 or bovine B4GALT1 genes, as indicated on the left-hand side of the panel.