Fig. 4.

N-glycosylation profiles. PSGs from (A), Flc-GG#1 (B), and Flc-GG#2 (C) silkworms were extracted, the extracts were used to produce total tryptic glycopeptides, and then total N-glycans were enzymatically released, permethylated, and analyzed by MALDI-TOF-MS, as described in Materials and methods. All molecular ions were detected as [M+Na]⁺, and peaks with signal to noise ratios of >3 were assigned and annotated with the standard cartoon symbolic representations.