In situ hybridization of AgamCPCFC1 on sections of pharate adults Animals were fixed 24 hours after pupation, which are a few hours before ecdysis to the adult. A. Hybridization to epidermis of sclerites (Scl), but not intersegmental membranes (IsM). B. Hybridization in muscle attachment region. C. Hybridization in spermatheca (Sp). D. Hybridization under basal plate (BP) of Johnston's organ, the surrounding pedicel (Ped) and the flagellum (Fl). E. Hybridization to part of cervical sclerite. (D,E 3’ probe; A,B,C coding region probe.)