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Western blot assay of TLR4 expression. On the 7th day after viral injection, the protein of lumbar spinal cord was prepared and the expression of TLR4 was detected. The protein expression of TLR4 in the Lv-siTLR4 with Dox group was also markedly downregulated compared to the other four groups receiving Walker 256 cells injection (∗ P < 0.01 versus Lv-siTLR4 group, Lv-MM group, NS group, and Dox group, one-way ANOVA analysis, N = 6).
