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. 2015 Oct 18;2015:475610. doi: 10.1155/2015/475610

Figure 4.

Figure 4

Effect of GHTT on mitochondrial membrane potential (a) and apoptosis (b) in Jurkat cells. (a) Jurkat cells were incubated with TMRM. After washing, the cells were treated with GHTT, DMSO, a negative control, and tunicamycin (Tm, 10 μg/mL), a positive control, followed by flow cytometry analysis. (b) Jurkat cells were incubated with GHTT and DMSO. After washing, the cells were stained with PI and annexin V and subjected to flow cytometry analysis. (c) Total lysates of Jurkat cells treated with GHTT and DMSO underwent SDS-PAGE. Following protein transfer, the membrane was blotted with the indicated antibodies.