Table 1.
Differentially expressed proteins in GHTT-treated Jurkat T cells identified using 2DE/MS analysis.
| ID | Protein name | Accession number | Theor. Mr (kDa) | Theor. pI | Sequence coverage (%) | Number of peptides matched | Avg. of fold differencea | Functional categorizationb | Identification method |
|---|---|---|---|---|---|---|---|---|---|
| GDIR2 | Rho GDP-dissociation inhibitor 2 | P52566 | 22974 | 5.10 | 41 | 5 | +4.0 | Signal transduction | MS, MS/MS |
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| GSTO1 | Glutathione S-transferase omega 1 | P78417 | 27548 | 6.23 | 44 | 12 | +2.0 | Detoxification | MS, MS/MS, ESI-MS/MS |
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| HBB | Hemoglobin beta chain | P68871 | 15871 | 7.26 | 15 | 4 | +3.4 | Transport | MS/MS |
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| PDIA3 | Protein disulfide isomerase A3 precursor | P30101 | 56747 | 5.98 | 28 | 15 | ++ | Metabolism | MS/MS |
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| ADA | Adenosine deaminase | P00813 | 40555 | 5.54 | 35 | 14 | +3.2 | Metabolism; energy pathways | MS, MS/MS |
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| SERA | D-3-phosphoglycerate dehydrogenase | O43175 | 56483 | 6.31 | 23 | 10 | +2.3 | Metabolism; energy pathways | MS, MS/MS |
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| ECHM | Enoyl-CoA hydratase, mitochondrial precursor | P30084 | 31379 | 8.34 | 33 | 7 | +2.9 | Metabolism; energy pathways | MS, MS/MS |
|
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| PARK7 | DJ-1 protein (oncogene DJ1) | Q99497 | 19878 | 6.33 | 42 | 7 | +1.6 | Nucleoside, nucleotide, and nucleic acid metabolism | MS, MS/MS |
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| IDHC | Isocitrate dehydrogenase [NADP] cytoplasmic | O75874 | 46630 | 6.53 | 28 | 9 | +1.7 | Metabolism; energy pathways | MS, MS/MS |
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| LTOR3 | Mitogen-activated protein kinase kinase 1 interacting protein 1 | Q9UHA4 | 13614 | 6.73 | 85 | 8 | +1.5 | Signal transduction | MS, MS/MS |
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| LMNB1 | LMNB1 protein | AAH78178 | 38118 | 5.37 | 26 | 9 | +5.1 | Miscellaneous | MS, MS/MS |
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| GSTP1 | Glutathione S-transferase P | P09211 | 23210 | 5.44 | 40 | 6 | +1.8 | Metabolism; energy pathways | MS, MS/MS, ESI-MS/MS |
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| VDAC2 | Voltage-dependent anion-selective channel protein 2 | P45880 | 38069 | 6.32 | 9 | 7 | +1.5 | Transport | MS, MS/MS |
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| RM39 | Mitochondrial 39S ribosomal protein L39 | Q9NYK5 | 34204 | 6.47 | 26 | 7 | — | Metabolism | MS, MS/MS |
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| MCCB | Methylcrotonoyl-CoA carboxylase beta chain | Q9HCC0 | 61294 | 7.57 | 31 | 12 | — | Metabolism; energy pathways | MS, MS/MS |
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| TXNL1 | Thioredoxin-like protein 1 | O43396 | 32100 | 4.84 | 58 | 10 | −4.4 | Metabolism | MS, MS/MS |
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| C3orf60 | Chromosome 3 open reading fragment 60 | AAH02873 | 20337 | 8.48 | 29 | 5 | −2.1 | Miscellaneous | MS, MS/MS |
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| BID | BH3 interacting domain death agonist | P55957 | 21981 | 5.27 | 41 | 7 | −3.8 | Apoptosis | MS, MS/MS |
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| PRDX3 | Thioredoxin-dependent peroxide reductase, mitochondrial precursor | P30048 | 27675 | 7.67 | 14 | 3 | −3.6 | Metabolism; energy pathways | MS/MS |
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| PPID | 40 kDa peptidyl-prolyl cis-trans isomerase | Q08752 | 40607 | 6.76 | 36 | 12 | −2.0 | Metabolism; energy pathways | MS, MS/MS, ESI-MS/MS |
|
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| GLRX3 | Thioredoxin-like protein 2 | O76003 | 37408 | 5.31 | 34 | 13 | −2.7 | Metabolism | MS, MS/MS, ESI-MS/MS |
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| GLRX3 | Thioredoxin-like protein 2 | O76003 | 37408 | 5.31 | 31 | 10 | −3.5 | Metabolism | MS, MS/MS, ESI-MS/MS |
|
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| NDUA5 | NADH-ubiquinone oxidoreductase 13 kDa-B subunit | Q16718 | 13319 | 5.76 | 49 | 7 | −2.1 | Metabolism; energy pathways | MS/MS |
aData were obtained from the analysis of protein spots (GHTT versus control) using PDQuest software. Symbol — represents that the respective protein spot in the GHTT gel was not detected.
bProteins were queried in Human Protein Reference Database (http://www.hprd.org/) for their functional categorization.