Table 2.
Linearity data of chromatographic HPTLC method for cucurbitacins B, D, and E; betulinic acid; picrosides 1 and 2; and apocynin (n = 3).
| S. number | Biomarkers | Solvent system | Linearity (ng spot−1) | Equation | Regression ± SD | Slope ± SD | Intercept ± SD | LOD (ng spot−1) | LOQ (ng spot−1) |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Cucurbitacin B | (Toluene : ethyl acetate : formic acid 60 : 40 : 0.5, v/v/v) | 66.7–833.3 | Y = 340.18 + 2.7x | 0.995 ± 0.001 | 2.70 ± 0.006 | 340.18 ± 0.040 | 22.06 | 66.86 |
| 2 | Cucurbitacin D | 166.6–833.33 | Y = 80.76 + 1.89x | 0.996 ± 0.001 | 1.89 ± 0.001 | 80.75 ± 0.020 | 54.85 | 166.20 | |
| 3 | Cucurbitacin E | 166.6–833.33 | Y = 200.09 + 2.33x | 0.998 ± 0.001 | 2.33 ± 0.006 | 200.76 ± 0.121 | 55.00 | 166.69 | |
| 4 | Betulinic acid | 250–2500 | Y = 827.64 + 0.504x | 0.993 ± 0.002 | 0.504 ± 0.004 | 827.64 ± 0.10 | 82.5 | 250.0 | |
|
| |||||||||
| 5 | Picroside 1 | (Toluene : ethyl acetate : methanol : formic acid 40 : 50 : 10 : 0.5, v/v/v/v) | 66.7–833.33 | Y = 565.75 + 5.00x | 0.994 ± 0.001 | 5.00 ± 0.002 | 565.8 ± 0.0133 | 21.97 | 66.58 |
| 6 | Picroside 2 | 67.7–833.33 | Y = 234.86 + 3.66x | 0.996 ± 0.002 | 3.66 ± 0.045 | 234.86 ± 0.303 | 22.28 | 67.53 | |
| 7 | Apocynin | 333.33–1666.66 | Y = 152.97 + 1.879x | 0.991 ± 0.001 | 1.87 ± 0.031 | 152.97 ± 1.25 | 110.18 | 333.88 | |