(a).
Parameters | Mean area ± SDb | % RSDc of area | |||
---|---|---|---|---|---|
Components | Conc. (ng spot−1) |
Wavelength used | |||
Detecting wavelengths (nma) 240 and 595 | Cucurbitacin B | 100 | 238 | 554.33 ± 11.67 | 2.10 |
242 | 545.66 ± 10.78 | 1.97 | |||
200 | 238 | 875.66 ± 19.55 | 2.23 | ||
242 | 876.66 ± 18.08 | 2.06 | |||
400 | 238 | 1594.66 ± 25.71 | 1.61 | ||
242 | 1588.66 ± 32.00 | 2.01 | |||
Cucurbitacin D | 200 | 238 | 441.0 ± 9.00 | 2.04 | |
242 | 438.33 ± 9.07 | 2.07 | |||
400 | 238 | 830.33 ± 16.55 | 1.98 | ||
242 | 824.33 ± 16.25 | 1.97 | |||
800 | 238 | 1648.00 ± 32.42 | 1.97 | ||
242 | 1650.33 ± 32.00 | 1.94 | |||
Cucurbitacin E | 200 | 238 | 640.66 ± 13.86 | 2.16 | |
242 | 638.00 ± 13.00 | 2.03 | |||
400 | 238 | 1173.33 ± 22.03 | 1.94 | ||
242 | 1169.33 ± 22.05 | 1.88 | |||
800 | 238 | 2161.33 ± 41.24 | 1.91 | ||
242 | 2154.33 ± 43.85 | 2.01 | |||
Betulinic acid | 250 | 593 | 981.33 ± 19.55 | 1.99 | |
597 | 977.33 ± 18.87 | 1.93 | |||
500 | 593 | 1039.66 ± 21.00 | 2.02 | ||
597 | 1031.00 ± 18.35 | 1.78 | |||
1000 | 593 | 1363.66 ± 27.09 | 1.98 | ||
597 | 1368.33 ± 27.61 | 2.01 | |||
| |||||
Detecting wavelength (nma) 500 | Picroside 1 | 100 | 498 | 941.33 ± 17.50 | 1.85 |
502 | 946.00 ± 17.34 | 1.83 | |||
200 | 498 | 1645.00 ± 34.05 | 2.06 | ||
502 | 1644.33 ± 32.86 | 1.99 | |||
400 | 498 | 2927.00 ± 55.05 | 1.88 | ||
502 | 2940.00 ± 56.66 | 1.92 | |||
Picroside 2 | 100 | 498 | 504.00 ± 10.00 | 1.98 | |
502 | 502.00 ± 9.53 | 1.90 | |||
200 | 498 | 1004.00 ± 20.42 | 2.03 | ||
502 | 1007.33 ± 19.50 | 1.93 | |||
400 | 498 | 1919.33 ± 37.01 | 1.92 | ||
502 | 1922.66 ± 40.69 | 2.11 | |||
Apocynin | 400 | 498 | 799.00 ± 15.50 | 1.94 | |
502 | 797.00 ± 14.54 | 1.82 | |||
800 | 498 | 1593.33 ± 29.36 | 1.84 | ||
502 | 1594.33 ± 31.65 | 1.98 | |||
1000 | 498 | 2227.33 ± 40.50 | 1.81 | ||
502 | 2257.00 ± 43.13 | 1.92 |
aNanometer. bStandard deviation. cRelative standard deviation.