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. Author manuscript; available in PMC: 2016 Oct 14.
Published in final edited form as: Cell Host Microbe. 2015 Oct 1;18(4):478–488. doi: 10.1016/j.chom.2015.09.002

Figure 1. Preservation of the inner mucus layer in histological sections allows for quantitative analysis of the spatial structure of the gut ecosystem.

Figure 1

(A) Schematic of protocol for gnotobiotic mouse experiments, tissue collection and processing, image acquisition, and analysis.

(B,C) Segments of mouse distal colon fixed in (B) 4% paraformaldehyde or (C) methacarn solution. Blue, DAPI staining in the epithelium; red, DAPI staining in lumen of gut, including bacteria and shed host nuclei (luminal debris has not been differentiated in this image); green, antibody staining for MUC2. Scale bars: 10 µm.