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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Calcif Tissue Int. 2015 Aug 29;97(6):611–623. doi: 10.1007/s00223-015-0055-9

Fig 3. CRFP treatment has dose dependent effects on the proliferation and osteogenic differentiation of adult mesenchymal stem cells.

Fig 3

A) Bar graph showing proliferation of adipose-derived hMSC in response to treatment with scrambled hCRFP and hCRFP at concentrations of 1, 5, and 10µM. Proliferation was measured on days 1, 3, 5, and 7 using an MTS assay and the results plotted as average ± SEM. Comparisons were made between groups at each time-point using Median Tests (SPSS) and considered significant for p-values < 0.05. Groups that differ are denoted with brackets. B) Images of hMSC stained with Alizarin to assess matrix mineralization (red), an indicator of osteogenic differentiation, following 8 and 12 days of treatment with scrambled hCRFP and hCRFP at concentrations of 1, 5, and 10µM. C) Bar graph showing quantitative results of the matrix mineralization assay as determined by eluting and measuring the amount of bound Alizarin spectrophotometrically. Comparisons were made between groups at each time-point using Median Tests (SPSS) and considered significant for p-values < 0.05. Groups that differ are denoted with brackets.