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. Author manuscript; available in PMC: 2016 Nov 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2015 Sep 5;289(1):58–69. doi: 10.1016/j.taap.2015.09.002

Fig. 2.

Fig. 2

Effects of CrVI on germ cell/oocyte apoptosis in cultured whole fetal ovaries on culture day 9. Fetal whole ovaries from embryonic day (E) 13.5 were cultured for 8 days in vitro, and treated with CrVI (0.1 ppm) from CD2 to CD8, harvested on CD9 (which recapitulated PND1), and used for histology (A-C) or TUNEL (D-F) or double immunolabeling of whole mount ovaries (G-I). The apoptosis index (AI) was calculated as the average percentage of TUNEL-positive germ cells/oocytes from 10 ovaries. Average number of TUNEL-positive cells in control group was considered as 10%. *: control vs CrVI, p<0.05. Each value represents mean ± SEM of TUNEL-positive germ cells/oocytes counted from 15 fields. The width of field for each image is 220 or 350 µm. In Fig 2C, arrow heads point out pyknotic nuclei and circle indicates primary follicle; in Fig 2F, arrow points out a primary follicle with TUNEL-positive (apoptotic) granulosa cells. In Figs 2G-I, arrow heads point out granulosa cells (red) and arrows point out germ cells or oocytes (green). GCN – germ cell nest, OVC - ovigerous cord, PF-primary follicle. FCO – freshly collected ovaries from PND1, CO – cultured ovaries on CD9. Representative images and histograms are shown.