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. Author manuscript; available in PMC: 2016 Nov 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2015 Sep 5;289(1):58–69. doi: 10.1016/j.taap.2015.09.002

Fig. 4.

Fig. 4

Effects of CrVI on the expression of BCL2, caspase3, BAX, p53 and PUMA in cultured whole fetal ovaries on culture day 9. Fetal ovaries from embryonic day (E) 13.5 were cultured for 8 days in vitro and treated with CrVI (0.1 ppm) from CD2 to CD8, and harvested on CD9 (which recapitulated PND1). Ovaries from culture were harvested and fixed in 4% buffered paraformaldehyde and processed for IHC. Integrated Optical Density (IOD) of staining was quantified using Image ProPlus software. The width of field for each image is 220 or 350 µm; BCL2 (A-C), caspase 3 (D-F), BAX (G-I), p53 (J-L) and PUMA (M-O). *: Control vs CrVI, p<0.05. Each value represents mean ± SEM of 10 ovaries. Representative images and histograms are shown.