Skip to main content
. Author manuscript; available in PMC: 2016 Nov 15.
Published in final edited form as: Bioorg Med Chem Lett. 2015 Sep 15;25(22):5349–5351. doi: 10.1016/j.bmcl.2015.09.036

Figure 1.

Figure 1

Photoaffinity-labeling of human GLO1 by biotinylated benzophenone-adenine photoprobe 1. (a) The structure of 1. (b) Photolabeling of recombinant human GLO1 (rhGLO1) with different concentrations of 1. 30 μL aliquots of rhGLO1 (0.05 μg/μl) in 1× Tris-buffered saline (TBS: 20 mM Tris-HCl, pH 7.4) were photolabeled with 1 at the final photoprobe concentrations of 73, 37, 18, 7 and 4 μM. Photocrosslinking was carried out under UV light (λmax 350 nm) for 2 h. See Supporting Information for more experimental details. (c) Photolabeling of rhGLO1 by 1 (7 μM) in the presence of excess (20×, 140 μM) adenine, benzophenone (4-methylbenzophenone), and biotin.