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The effect of ketoprofen binding on the catalytic activity of rhGLO1. (a) The Dixon plot of quercetin, a known inhibitor of GLO1 (positive control). Quercetin gave a Ki value of 1.69 ± 0.14 μM in this assay. (b) The Dixon plot of ketoprofen. The initial substrate concentrations were (◆) 400 μM, (■) 200 μM, (▲) 100 μM, and (×) 50 μM.
