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. 2015 Aug 12;309(9):C593–C599. doi: 10.1152/ajpcell.00069.2015

Fig. 1.

Fig. 1.

Mouse coronary endothelial cells (MCECs) isolated from diabetic (D) mice exhibit decreased β-N-acetylglucosaminidase (OGA) protein levels and increased protein O-linked N-acetylglucosaminylation (O-GlcNAcylation) compared with endothelial cells (ECs) from control (C) mice. A: Western blots showing OGA and actin protein levels (top). Actin was used as a loading control. Bottom: OGA protein level normalized to actin. Values are means ± SE; n = 8 per group. *P < 0.05 vs. control. B: Western blots showing O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) and actin protein levels (top). Bottom: OGT protein level normalized to actin. Values are means ± SE; n = 7 per group. C: increased protein O-GlcNAcylation was detected in diabetic ECs with the anti-O-GlcNAc antibody RL2. Arrow indicates a representative band of proteins showing increased O-GlcNAcylation. D: summarized data of the intensity of the band indicated by the arrow (left) and total RL2 signals (right). Values are means ± SE; n = 6 per group. *P < 0.05 vs. control.