Fig. 2.

Generation of tetracycline (Tet)-inducible OGA transgenic (Tie2-Tet-OGA) mice. A: Tie2-rtTA mice were crossed with TRE-OGA mice to generate Tie2-Tet-OGA mice. rtTA, reverse Tet-controlled transactivator; TRE, Tet response element; CMV, cytomegalovirus promoter; Dox, doxycycline. B: genotyping of transgenic mice by PCR. Lane 4 carries both OGA and rtTA transgenes. C: Dox treatment in Tie2-Tet-OGA mice (lane 4) significantly increased OGA mRNA in coronary ECs. OGA mRNA expression level was determined by real-time PCR. Values are means ± SE; n = 6 per group. *P < 0.05 vs. untreated [without Dox (−Dox)]. D: Dox treatment in Tie2-Tet-OGA mice significantly increased OGA protein expression (n = 3 −Dox and 5 +Dox) but decreased OGT protein levels (n = 3 per group), as determined by Western blot analysis. Values are means ± SE. *P < 0.05 vs. −Dox.