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. 2015 Aug 12;309(9):C593–C599. doi: 10.1152/ajpcell.00069.2015

Fig. 5.

Fig. 5.

Effect of OGA overexpression on connexin 40 (Cx40) protein expression and O-GlcNAcylation in diabetic mice. A: immunoprecipitation (IP) with anti-RL2 antibody and immunoblotting (IB) with anti-Cx40 antibody (top) and Western blots (WB) of Cx40 and actin protein levels (bottom). B: O-GlcNAcylated Cx40 protein levels in MCECs isolated from control, untreated diabetic, and Dox-treated diabetic mice. Values are means ± SE; n = 3 per group. *P < 0.05 vs. control; #P < 0.05 vs. diabetic −Dox. C: Cx40 protein levels in MCECs isolated from control, untreated diabetic, and Dox-treated diabetic mice. Values are means ± SE; n = 4 per group. D: IP with anti-RL2 antibody and IB with anti-eNOS antibody (top) and Western blots of eNOS and actin protein levels (bottom). E: O-GlcNAcylated endothelial nitric oxide synthase (eNOS) protein levels in MCECs isolated from control, untreated diabetic, and Dox-treated diabetic mice. Values are means ± SE; n = 3 per group. *P < 0.05 vs. control. F: eNOS protein levels in MCECs isolated from control, untreated diabetic, and Dox-treated diabetic mice. Values are means ± SE; n = 4 per group.