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. 2015 Oct 19;2015:416838. doi: 10.1155/2015/416838

Table 2.

Information regarding the genotyping of tag SNPs in candidate genes.

Gene Tag SNP Major > minor Sequence of primers (5′ to 3′) Annealing temperature (°C) Cycle number Analytical method (restriction enzyme)
Forward Reverse
IL17A rs4711998 A > G TGTCCTCCAATTCCCTTTTG GAGACATGATGGGGGAAAGA 60 30 PCR-RFLP (Bpu E I)
rs8193036 C > T TCTTTCCCCCATCATGTCTC GTTCCAACCCTGCATGCTAC 60 30 PCR-direct DNA sequencing
rs3819024 G > A AGGCACAAACTCATCCATCC GTCAGAACCCAGCGTTTCAT 60 30 PCR-direct DNA sequencing
rs2275913 G > A GTTTCCGGAATTGTCTCCAC CCCAGGAGTCATCGTTGTTT 60 30 PCR-RFLP (Bsl I)
rs3819025 A > G AGTTTCCGGAATTGTCTCCA CAGCTGCCAGAAGAGTTATGC 60 30 PCR-direct DNA sequencing

IL17F rs763780 T > C CACTGGTGCTCTGATGAGGA CTGCATCAATGCTCAAGGAA 60 35 PCR-RFLP (Hsp 92 II)
rs766748 G > A GTGGGAATGAGTGGAGGAGA TCCACTCGCTAAGCTGGACT 60 30 PCR-RFLP (Afa I)
rs12201582 C > A ACATTACGCAAAACCAACGA TCATCCCCAGTAAGGGTCAG 60 30 PCR-direct DNA sequencing
rs9382084 T > G ATCAACTTTCATCCCCCACA CGCCATAGCAGTTTGTCAAG 60 35 PCR-RFLP (Alu I)
rs722323 C > T GGATTTCTCTGAGAGGTGCTG GGAAATCAGATGATGTCTGCAA 60 30 PCR-direct DNA sequencing
rs1266828 T > C CCCTGGATGGAAGAAATGAA CATCAAAGCCTATGCCCCTA 60 30 PCR-RFLP (Hpy CH4 V)
rs2294834 A > C TTTGATTGGGGTCTTTTTGG GGGATTACAGGCAACTGACC 60 30 PCR-direct DNA sequencing

IL17RA rs2270241 T > G CTGCGACTCCTGGACCAC GTATTCCACACCGCAACTCC 60 35 PCR-direct DNA sequencing
rs2241042 A > C AGCTGCTTGCACAACTGCTA CGCTTCTGGCATCTTTCTC 60 30 PCR-RFLP (Hha I)
rs5748863 G > A CAGGCGTGAGCCATGAAATT GGCGCAGGGATCTACTGTTT 61 35 PCR-RFLP (Bsa A I)
rs5748864 G > A CTTGGTCTGGGTCTTCGTTG GGAACCTCCACATGTTCCAC 60 30 PCR-RFLP (Hpy CH4 IV)
rs2241044 A > C TATGGGAACCAGAGCACCTC GGTCCCAGGATGAAGAAGGT 60 35 PCR-RFLP (Tsp R I)
rs2241049 A > G ATGACCCTAGGCTGCTCCTT GCGGGGGTTAACTCCTTAGT 60 30 PCR-RFLP (Hpy 188 I)
rs2229151 G > A TCATCTACTCAGCCGACCAC GGAGCACAGGACGATGATCT 60 30 PCR-RFLP (Hpy 188 III)
rs2895332 A > G GCGTCCTTGAGGCTCCATTA CTGGCCCATTCAGCGTTTAC 60 30 PCR-RFLP (Hpy CH4 IV)

IL17RC rs7627880 G > A CAAGGTCTCTTGTGCTTGC GGATGCACTCATTCAGCAG 58 30 PCR-RFLP (Mse I)
rs279545 A > G CAGCCCTGGGAAAGTTAAG CTGTCAAGATCCCCACTCC 60 30 PCR-RFLP (Bse Y I)

TRAF3IP2 rs10872070 T > C CAAGCCTAGGCCATAAGCAG TCCCACGTAGTCACCATTCA 60 30 PCR-RFLP (Hsp 92 II)
rs6941014 T > C AGCAGAGGGTGAGAGCATGT TTGCTGATGAGCCTGAGATG 60 30 PCR-RFLP (Hae III)
rs1883136 C > A AATAGCTTCCCTGCGGACTT GTGGGAGTTCCTGCAACAGT 60 30 PCR-RFLP (Ssp I)
rs1040383 C > G AGGCAACCAACTGGCAATAC CTTTCTCCCAGGTTGCACAT 60 30 PCR-RFLP (Xsp I)
rs9374263 G > C TTTAACCAGGCCCACATGAT TCAGGAGAGGAGCTGTTGGT 60 30 PCR-RFLP (Hae III)
rs2075966 A > G GAAATTGGCGATGGTATTGG TCACACCTCCAGACATTTGC 60 30 PCR-RFLP (Xsp I)

SNP: single nucleotide polymorphism; 3′-UTR: 3′ untranslated region; PCR: polymerase chain reaction; RFLP: restriction fragment length polymorphism.