Table 2.
Gene | Tag SNP | Major > minor | Sequence of primers (5′ to 3′) | Annealing temperature (°C) | Cycle number | Analytical method (restriction enzyme) | |
---|---|---|---|---|---|---|---|
Forward | Reverse | ||||||
IL17A | rs4711998 | A > G | TGTCCTCCAATTCCCTTTTG | GAGACATGATGGGGGAAAGA | 60 | 30 | PCR-RFLP (Bpu E I) |
rs8193036 | C > T | TCTTTCCCCCATCATGTCTC | GTTCCAACCCTGCATGCTAC | 60 | 30 | PCR-direct DNA sequencing | |
rs3819024 | G > A | AGGCACAAACTCATCCATCC | GTCAGAACCCAGCGTTTCAT | 60 | 30 | PCR-direct DNA sequencing | |
rs2275913 | G > A | GTTTCCGGAATTGTCTCCAC | CCCAGGAGTCATCGTTGTTT | 60 | 30 | PCR-RFLP (Bsl I) | |
rs3819025 | A > G | AGTTTCCGGAATTGTCTCCA | CAGCTGCCAGAAGAGTTATGC | 60 | 30 | PCR-direct DNA sequencing | |
| |||||||
IL17F | rs763780 | T > C | CACTGGTGCTCTGATGAGGA | CTGCATCAATGCTCAAGGAA | 60 | 35 | PCR-RFLP (Hsp 92 II) |
rs766748 | G > A | GTGGGAATGAGTGGAGGAGA | TCCACTCGCTAAGCTGGACT | 60 | 30 | PCR-RFLP (Afa I) | |
rs12201582 | C > A | ACATTACGCAAAACCAACGA | TCATCCCCAGTAAGGGTCAG | 60 | 30 | PCR-direct DNA sequencing | |
rs9382084 | T > G | ATCAACTTTCATCCCCCACA | CGCCATAGCAGTTTGTCAAG | 60 | 35 | PCR-RFLP (Alu I) | |
rs722323 | C > T | GGATTTCTCTGAGAGGTGCTG | GGAAATCAGATGATGTCTGCAA | 60 | 30 | PCR-direct DNA sequencing | |
rs1266828 | T > C | CCCTGGATGGAAGAAATGAA | CATCAAAGCCTATGCCCCTA | 60 | 30 | PCR-RFLP (Hpy CH4 V) | |
rs2294834 | A > C | TTTGATTGGGGTCTTTTTGG | GGGATTACAGGCAACTGACC | 60 | 30 | PCR-direct DNA sequencing | |
| |||||||
IL17RA | rs2270241 | T > G | CTGCGACTCCTGGACCAC | GTATTCCACACCGCAACTCC | 60 | 35 | PCR-direct DNA sequencing |
rs2241042 | A > C | AGCTGCTTGCACAACTGCTA | CGCTTCTGGCATCTTTCTC | 60 | 30 | PCR-RFLP (Hha I) | |
rs5748863 | G > A | CAGGCGTGAGCCATGAAATT | GGCGCAGGGATCTACTGTTT | 61 | 35 | PCR-RFLP (Bsa A I) | |
rs5748864 | G > A | CTTGGTCTGGGTCTTCGTTG | GGAACCTCCACATGTTCCAC | 60 | 30 | PCR-RFLP (Hpy CH4 IV) | |
rs2241044 | A > C | TATGGGAACCAGAGCACCTC | GGTCCCAGGATGAAGAAGGT | 60 | 35 | PCR-RFLP (Tsp R I) | |
rs2241049 | A > G | ATGACCCTAGGCTGCTCCTT | GCGGGGGTTAACTCCTTAGT | 60 | 30 | PCR-RFLP (Hpy 188 I) | |
rs2229151 | G > A | TCATCTACTCAGCCGACCAC | GGAGCACAGGACGATGATCT | 60 | 30 | PCR-RFLP (Hpy 188 III) | |
rs2895332 | A > G | GCGTCCTTGAGGCTCCATTA | CTGGCCCATTCAGCGTTTAC | 60 | 30 | PCR-RFLP (Hpy CH4 IV) | |
| |||||||
IL17RC | rs7627880 | G > A | CAAGGTCTCTTGTGCTTGC | GGATGCACTCATTCAGCAG | 58 | 30 | PCR-RFLP (Mse I) |
rs279545 | A > G | CAGCCCTGGGAAAGTTAAG | CTGTCAAGATCCCCACTCC | 60 | 30 | PCR-RFLP (Bse Y I) | |
| |||||||
TRAF3IP2 | rs10872070 | T > C | CAAGCCTAGGCCATAAGCAG | TCCCACGTAGTCACCATTCA | 60 | 30 | PCR-RFLP (Hsp 92 II) |
rs6941014 | T > C | AGCAGAGGGTGAGAGCATGT | TTGCTGATGAGCCTGAGATG | 60 | 30 | PCR-RFLP (Hae III) | |
rs1883136 | C > A | AATAGCTTCCCTGCGGACTT | GTGGGAGTTCCTGCAACAGT | 60 | 30 | PCR-RFLP (Ssp I) | |
rs1040383 | C > G | AGGCAACCAACTGGCAATAC | CTTTCTCCCAGGTTGCACAT | 60 | 30 | PCR-RFLP (Xsp I) | |
rs9374263 | G > C | TTTAACCAGGCCCACATGAT | TCAGGAGAGGAGCTGTTGGT | 60 | 30 | PCR-RFLP (Hae III) | |
rs2075966 | A > G | GAAATTGGCGATGGTATTGG | TCACACCTCCAGACATTTGC | 60 | 30 | PCR-RFLP (Xsp I) |
SNP: single nucleotide polymorphism; 3′-UTR: 3′ untranslated region; PCR: polymerase chain reaction; RFLP: restriction fragment length polymorphism.