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. 2015 Sep 4;309(9):L970–L982. doi: 10.1152/ajplung.00041.2015

Fig. 3.

Fig. 3.

Effects of long-chain free fatty acids (oleic acid or linoleic acid) or a synthetic agonist of both FFAR1 and FFAR4 (GW9508) on intracellular Ca2+ concentrations ([Ca2+]i) in HASM cells. A: representative traces of fluorescence intensity [change in fluorescence (ΔF) from baseline fluorescence (Fo)] illustrating the characteristics of oleic acid (i; 10 μM)-, linoleic acid (ii; 10 μM)-, or GW9508 (iii; 10 μM)-induced [Ca2+]i increases in HASM cells. At time 0, oleic acid, linoleic acid, or GW9508 was injected into the buffer in the wells. B: concentration-dependent effects (0.1–10 μM) of oleic acid, linoleic acid, or GW9508 on transient (peak) fluorescence increases in HASM cells. Data are means ± SE presented as ΔF/Fo; n = 4–11. C: effects of oleic acid (10 μM), linoleic acid (10 μM), GW9508 (10 μM), vehicle (0.1% DMSO), bradykinin (1 μM), acetylcholine (1 μM), or histamine (10 μM) on transient (peak) fluorescence increases in HASM cells. Data are means ± SE presented as ΔF/Fo; n = 5–11 (shown in parentheses).