Effects of pretreatment with U-73122 (5 μM), xestospongin C (Xest C; 20 μM), ryanodine (100 μM), or pertussis toxin (PTX; 100 ng/ml) on peak increase in [Ca2+]i stimulated by ligands for FFAR1/FFAR4 (oleic acid, linoleic acid, or GW9508; 10 μM each) in HASM cells. Data are means ± SE presented as a percentage of the ligand (10 μM)-stimulated fluorescence increases in the absence of inhibitors. *P < 0.05; **P <0.01; ***P < 0.001 compared with FFAR1/FFAR4 ligands (10 μM) alone. Numbers of experiments are shown in parentheses.