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. 2015 Aug 28;309(9):L1009–L1017. doi: 10.1152/ajplung.00386.2014

Fig. 1.

Fig. 1.

Effect of oxygen tension and ATP on the interaction of endothelial nitric oxide synthase (eNOS) with hsp90 and porin in mitochondria. Endothelial NOS was immunoprecipitated (IP) from mitochondrial isolates of pulmonary artery endothelial cells (PAEC) exposed to 3, 21, or 95% O2 for 1 h after being cultured in 3% O2 (A). IB, immunoblot. Representative blot and summary data (B) show increase in eNOS interactions with client proteins after transition to 21% and decrease with 95% O2 (means ± SD for n = 5). ATP also stimulated increases in eNOS interactions with porin and hsp90 as shown in representative blot (C) and summary data (D, for n = 5). *P < 0.05, from 3% O2 (B) or from basal level (D) by ANOVA and Newman Keuls post hoc test.