Fig. 2.
Effect of transition from fetal (3%) to normoxic (21%) or hyperoxic (95%) oxygen tension on mitochondrial targeted hydroethidine (Mito-HE) fluorescence. The increase in fluorescence by hyperoxia was quenched by cell permeable polyethylene glycol superoxide dismutase (SOD) at 100 U/ml, indicating that reactive oxygen species contribute to increase in fluorescence. Summary data are shown on the right as means ± SD for 5 experiments. *P < 0.05, from 3% O2 by ANOVA and Newman-Keuls post hoc test.