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. 2015 Aug 28;309(9):L1009–L1017. doi: 10.1152/ajplung.00386.2014

Fig. 4.

Fig. 4.

Effect of decoy peptides on NO and mitochondrial ROS levels and O2 consumption by PAEC. Increase in NO2− + NO3− levels in response to ATP was attenuated by decoy peptides targeting eNOS-porin (PDP) or eNOS-Hsp90 (TSB2) interactions (A). l-NMMA (10-4M) attenuated the increase in NO2− + NO3− levels in response to ATP, indicating that NO release accounts for the increase in NO2− + NO3− levels. PDP increased Mito-HE fluorescence at basal level and both TSB2 and PDP increased the fluorescence in response to ATP (B). Control peptides had no effect on basal O2 consumption (C); both decoy peptides (10−5 M) and NG-nitro-l-arginine methyl ester (l-NAME; 10−4M) increased the O2 consumption rate from basal level. *P < 0.05, from basal level for n = 6 for all 3 studies.