Fig. 7.
Effect of decoy peptides blocking eNOS interactions with porin (PDP; A) and Hsp90 (TSB2; B) on PAEC migration into scratch margins. The frontline gap is indicated by broken lines in the center. Both PDP and TSB2 reduced PAEC migration into the scratch area, increasing gap distance. NAC (10−4 M) and Mito-Q (10−9 M) restored the migration of cells, suggesting that oxidative stress contributed to impaired cell migration with decoy peptides. Summarized data are shown for front line gap (C) as means ± SD from 6 experiments. Both PDP and TSB2 also increased apoptosis of PAEC (D). NAC and Mito-Q attenuated the apoptosis caused by the decoy peptides. *P < 0.05, from control peptide by ANOVA and Newman Keuls test.