Fig. 11.

Cortactin SNP impairs endothelial barrier enhancement by S1P. Here S1P was added at t = 0, and the traces show the endothelial resistance as a function of time after S1P addition. HPAECs were transfected with green fluorescent protein (GFP) vector (orange line), WT GFP-cortactin (blue line), or SNP GFP-cortactin (red line) as described in materials and methods. Black line represents control HPAECs treated with vehicle only. Transendothelial electrical resistance (TER) traces represent pooled results ± SE from n = 5–8 independent experiments per condition. The maximal resistance level of WT GFP-cortactin was higher than that of SNP GFP-cortactin (P < 0.04). GFP vector-transfected HPAEC values (orange) are shown without SE bars as a reference. Mean baseline TER values for each conditions are as follows: vehicle control (no transfection) = 1,126 Ω, GFP vector control = 990 Ω, WT GFP-cortactin = 1,019 Ω, SNP GFP-cortactin = 915 Ω.