Fig. 4.

Formation and disappearance of actin belts around gaps. A: gaps on WT cortactin- and cortactin SNP-overexpressing BAEC monolayers, showing actin stained with Alexa Fluor 488-phalloidin just after large gap formation (t = 0 min). This actin belt morphology was found around both small gaps as well. B and C: actin belt morphology around small gaps (B) and large gaps (C) in a BAEC monolayer overexpressing cortactin SNP. Samples fixed at t = 0 min and 25 min. After the actin belt disappears, small gaps close by cell rearrangement and large gaps close by lamellipodial extension and cell migration (Supplemental Videos S1–S6). This trend was also seen with WT cortactin. Scale bars, 20 μm.