Table 1.
The primers used in real-time PCR.
| Gene | GenBank | Primers | |
|---|---|---|---|
| MS | NM_030864.1 | F | 5′-GGATCTCTGGGTCCGACTAACAA-3′ |
| R | 5′-GCCTGCTCCTGGTATGCTTCA-3′ | ||
|
| |||
| BHMT | NM_030850.1 | F | 5′-GAACCAGAGTTGCCACCAGATG-3′ |
| R | 5′-AGCAGCCGCCAATGTACCTGAC-3′ | ||
|
| |||
| CBS | NM_012522.2 | F | 5′-AAGGCTGCCCAGGAGCTAAG-3′ |
| R | 5′-CAGCATCCATTTGTCACTCAAGAAC-3′ | ||
|
| |||
| CSE | NM_017074.1 | F | 5′-GTCCACAAACACAAAGACATCA-3′ |
| R | 5′-AGGTCATCGGAAGTAACAGACA-3′ | ||
|
| |||
| β-actin | NM_007393.1 | F | 5′-GTCCCTCACCCTCCCAAAA-3′ |
| R | 5′-GCTGCCTCAACACCTCAACCC-3′ | ||
MS, methionine synthase; BHMT, betaine-homocysteine methyltransferase; CBS, cystathionine β-synthase; CSE, cystathionine γ-lyase; F, forward; R, reverse.