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. 2015 Sep 1;47(11):559–568. doi: 10.1152/physiolgenomics.00062.2015

Fig. 2.

Fig. 2.

Analysis of recombinant cell lines incorporating the rat Igf1 locus. A: experimental plan to test responsiveness to Stat5b of the rat Igf1 gene in individual BAC-containing cell lines (Dox, doxycycline). B: results of RT-PCR experiments for rat Igf1 (primers span exons 3 and 4) or mouse Gapdh mRNA (see Table 2) using RNA isolated from the listed recombinant cell lines transduced with constitutively active rat Stat5b and tTA and treated ± Dox for 18 h. C: experimental plan to test GH responsiveness of the rat Igf1 gene in individual BAC-containing cell lines. D: results of RT-PCR experiments using RNA isolated from the listed recombinant cell lines transduced with the mouse GH receptor, rat Stat5b, and tTA, and treated with vehicle or recombinant rat GH [40 nM] for 18 h. Liver RNA from GH-deficient rats treated with vehicle or recombinant rat GH [1.5 mg/kg] for 6 h represents a negative and positive control respectively for GH responsiveness.