Fig. 4.

Rapid induction of Igf1 gene transcription by GH and Stat5b in a BAC-containing cell line. A: representative experiment showing time course of appearance of nascent transcripts for the rat Igf1 gene and for several mouse genes, as assessed by RT-PCR using nuclear RNA isolated at different times after GH addition to clone 11 cells. B: quantitative analysis of time course of accumulation of nascent transcripts from rat Igf1 promoters 1 and 2 and for mouse Igfals, Socs2, and Gapdh, measured at different times after addition of GH to clone 11 cells, as assessed by qRT-PCR. Primer sets are listed in Table 3 (means ± SD of n = 5 independent experiments; *P < 0.01; **P < 0.001 vs. time 0).