Fig. 5.

GH induces recruitment of Stat5b, RNA polymerase II, and p300 to potential binding elements in chromatin within the rat Igf1 locus. Results are shown of quantitative chromatin immunoprecipitation experiments using chromatin harvested from rat Igf1-BAC clone 11 after addition to cells of recombinant rat GH [40 nM] for 0, 1 or 4 h. Primer sets are listed in Table 4. All studies were performed by qPCR. A: analysis of binding of Stat5b to the 7 Stat5 binding elements depicted in Fig. 1 plus control primer pairs dispersed throughout the rat Igf1 locus (Neg). B: results of binding of RNA polymerase II (pol II) to the same elements. C: binding of p300 to the same elements. Results reflect the means ± SD of 3–5 independent experiments (*P < 0.05; **P < 0.01; ***P < 0.001 vs. time 0).