Table 3.
Primers used for quantitative RT-PCR of nuclear RNA
| Gene | Location | DNA Sequence | Amplicon, bp |
|---|---|---|---|
| Rat Igf1 | exon 1 | 5′-CTGCCTCTGTGACTTCTTGAAGG | 224 |
| intron 1 | 5′-AATGCATTCATCTCCCGGACCTC | ||
| Rat Igf1 | exon 2 | 5′-GTACCAAAATGAGCGCACCTCCA | 316 |
| intron 2 | 5′-CTCCCCATATGTTCTCCATTCCC | ||
| Mouse | exon 2 | 5′-GATAGCATCCCAGTCAGCA | 189 |
| Igfals | intron 2 | 5′-GTTCCGTTCCAGGTGCAGAT | |
| Mouse | exon 2 | 5′-GAATGGAGCGGACAGGAC | 122 |
| Socs2 | intron 2 | 5′- CCAGCTCCCTACCTGTTTGA | |
| Mouse | exon 2 | 5′-AGGAGCGAGACCCCACTAAC | 114 |
| Gapdh | intron 3 | 5′-CAGCTTTCCGGCCACTTAC |