Table 4.
DNA sequences of Igf1 locus oligonucleotide primers for ChIP assays
| Location (from 5′ end) | Element | Top Strand (5′ to 3′) | Bottom Strand (5′ to 3′) | Amplicon, bp |
|---|---|---|---|---|
| −86264 | R2-4 | TGAATCTCCAGGGATCACACCA | CTCTTGTTGATTTCATCTTTGACCCA | 84 |
| −73096 | R8-9 | GTGAGTCTGTGTTAGTCAGG | GGTTATGTAAGTCTGATAGAG | 93 |
| −62806 | R13 | TGCATTGGCTACCAGGAACTCT | GAGACAGTTGGAAGGAAGGATAGA | 76 |
| +3740 | R34-35 | GGTGAAACCGCTCACCTTGG | TTCTAAGAAGCAGACAGAGG | 103 |
| +26694 | R53-54 | TGGCACATGCCATTGACCAGAT | AAGGGCAGACCCATCTTTCTGA | 102 |
| +43817 | R57-59 | GGCCAATTCATTCGGCAACTGT | TCCTTTCTGAGAACTGGAGACCGT | 87 |
| +49147 | R60-61 | CCCAACTGAGAAGTAGTAGGCT | GAAACAGTCCCGACAACCTATC | 105 |
| −53692 | Neg1 | TGACAGTAGTCCGAGGCTCATT | ACCGTGAGATTTCTTAGGTGGTGC | 82 |
| −10054 | Neg2 | CCAGCCGTTAAACACCAAGAAG | TGCTAGTCCCAGTACAGCATCT | 94 |
| +12555 | Neg3 | AGCGTTCTTCCAAACACCTTGTGC | GGTTGGCTCTGATGGAGTATCT | 75 |
ChIP, chromatin immunoprecipitation.